Study of chemical composition and antimicrobial properties of Rosemary (Rosmarinus officinalis) essential oil on Staphylococcus aureus and Escherichia coli in vitro

Authors : Abolfazl JAFARI-SALES, Mehrdad PASHAZADEH

Pages : 62-69

Doi:10.38001/ijlsb.693371

View : 14 | Download : 6

Publication Date : 2020-04-15

Article Type : Research Paper

Abstract :Nowadays, with increasing bacterial resistance, finding new antimicrobial agents to replace herbal remedies with less side effects than conventional drugs has provided. The aim of this study was to investigate the chemical composition and antimicrobial properties of Rosemary insert ignore into journalissuearticles values(Rosmarinus officinalis); essential oil on Staphylococcus aureus and Escherichia coli in vitro. In this experimental study, rosemary was prepared from Tabriz city and the essential oil was extracted after drying by water distillation using Clevenger apparatus. The essential oil components were identified by Gas chromatography mass spectrometry insert ignore into journalissuearticles values(GC-MS);. Essential oil of 10, 5, 2.5, 1.25, 0.625 and 0.312% of essential oil was used to determine minimum inhibitory concentration insert ignore into journalissuearticles values(MIC); and minimum bactericidal concentration insert ignore into journalissuearticles values(MBC);. Based on the results of GC-MS analysis, 19 compounds were identified, of which 1.8 Cineole and α-pinen had the highest essential oil composition. MIC and MBC of essential oils were obtained on S. aureus insert ignore into journalissuearticles values(0.625 and 1.25%); and E. coli insert ignore into journalissuearticles values(1.25 and 2.5%);, respectively. The results of this study showed that rosemary essential oil has remarkable antibacterial activity and can be used as a suitable replacement for synthetic antibiotics.
Keywords : Antibacterial effects, Essential oils, Rosemary, Pathogenic bacteria