Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana

Authors : Meltem BAYRAKTAR, Elmira NAZİRİ, Fatih KARABEY, İsmail Hakki AKGUN, Erdal BEDİR, Bärbel RÖCK-OKUYUCU, Aynur GÜREL

Pages : 362-374

Doi:10.21448/ijsm.496724

View : 23 | Download : 6

Publication Date : 2018-12-29

Article Type : Research Paper

Abstract :Stevia rebaudiana Bertoni, which is an important plant for the food and health sector, contains calorie-free natural sweet-tasting steviol glycosides insert ignore into journalissuearticles values(SGs);. In the present study, the effects of different elicitors [methyl jasmonate insert ignore into journalissuearticles values(MeJA);, salicylic acid insert ignore into journalissuearticles values(SA);, or chitosan insert ignore into journalissuearticles values(CHI);] on the in vitro production of stevioside and rebaudioside A were carried out. For this purpose, 3-week-old in vitro plantlets were transferred into 250 mL flasks containing liquid woody plant medium insert ignore into journalissuearticles values(WPM); supplemented with MeJA, SA, or CHI at different concentrations insert ignore into journalissuearticles values(0, 50, 100, or 200 µM);, and were exposed to these elicitors for 2 weeks. A HPLC method was developed to quantify the aforementioned SGs in the cultivated plantlets and all of the elicitor types and concentrations resulted in an increase in stevioside production ranged between 2.87 mg/g dry weight insert ignore into journalissuearticles values(DW); insert ignore into journalissuearticles values(Control); and 50.07 mg/g DW insert ignore into journalissuearticles values(100 µM MeJA);. The highest number of shoot, node, leaf, leaf length, and biomass accumulation and shoot length were observed with application of 100 µM CHI and control, respectively. The present findings open new perspectives for increasing the stevioside production using a plant tissue culture system.
Keywords : Chitosan, Elicitor, Methyl jasmonate, Salicylic acid, Steviol glycosides, Stevia rebaudiana