Adventitious roots formation for enhanced and sustainable production of antioxidants in Brassica oleracea var. acephala (Brassicaceae)

Authors : Muhammad ADİL, Bilal Haider ABBASİ

Pages : 162-171

Doi:10.21448/ijsm.530027

View : 9 | Download : 7

Publication Date : 2019-07-15

Article Type : Research Paper

Abstract :Brassica oleracea var. acephala is listed as the healthiest vegetable due to its high valued secondary metabolites content and antioxidant potential. This study was conducted to establish adventitious roots insert ignore into journalissuearticles values(ARs); culture as an alternative and feasible production of antioxidant secondary metabolites. ARs were induced from cotyledon explants in commercially available Murashige and Skoog insert ignore into journalissuearticles values(MS); plant nutrient media, gelled with 0.8% phyto-agar and supplemented with different concentration insert ignore into journalissuearticles values(0.1 ̶ 1.5 mg·L-1); of auxins insert ignore into journalissuearticles values(α-Naphthalene acetic acid; NAA, or Indole acetic acid; IAA, or Indole-3-butyric acid; IBA);. AR formation responses in MS media at varying concentrations insert ignore into journalissuearticles values(0 ̶ 50 g·L-1); of sucrose and initial media pH insert ignore into journalissuearticles values(4, 5.0, 5.8, 7 & 8); were also studied. The bioprocessing of ARs were studied in liquid MS media containing NAA insert ignore into journalissuearticles values(1.5 mg·L-1); as growth regulator. The growth curve, important antioxidants insert ignore into journalissuearticles values(phenols & flavonoids);, and free radical scavenging potential of ARs were studied for a period of 9-weeks. The ARs at stationary phase insert ignore into journalissuearticles values(7-week); attained highest accumulation of phenols and flavonoids, which ultimately showed the highest reactive species scavenging potential. This study provides the base for production of B. oleraceae var. acephala secondary metabolites on large scale to strengthen the bio-based economy of developing world.
Keywords : Adventitious roots, Phenolics, Flavonoids, Antioxidants, Auxin