Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins

Authors : Valentina MURSALIYEVA, Akjunus IMANBAYEVA

Pages : 1-7

Doi:10.21448/ijsm.627140

View : 17 | Download : 7

Publication Date : 2020-03-20

Article Type : Research Paper

Abstract :Allochrusa gypsophiloides or Turkestan soap root insert ignore into journalissuearticles values(TSR);, which is endemic to Central Asia, is a valuable saponin-bearing technical and medicinal plant. The purpose of this study was to develop in vitro mass propagation for the conservation of endangered species. Node explants were induced to regenerate adventitious shoot buds on Murashige and Skoog medium insert ignore into journalissuearticles values(MS); supplemented with different concentrations of 6-benzylaminopurine insert ignore into journalissuearticles values(BAP); and/or kinetin in combination with naphtylacetic acid insert ignore into journalissuearticles values(NAA);. The maximum number of shorter shoots per explant insert ignore into journalissuearticles values(16.8 ± 3.1); was recorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one month cultivation. For elongation, obtained shoot conglomerates were transfered for hormone-free MS. The cultivation of initial explants on MS with kinetin led to a three-fold reduction in the number of shoots characterized by a maximum length and clearly defined internodes insert ignore into journalissuearticles values(without the stage of elongation);. Micropropagation was achieved by cutting obtained shoots and adventitious shoot induction. The maximal shoot proliferation insert ignore into journalissuearticles values(62 ± 6.9); on MS 0.5 mg/L BAP + 1.0 mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required to elongation before rooting on the liquid ½ MS medium contained NAA or IBA. In both cases, auxin concentration 2.0 mg/L induced maximal rooting insert ignore into journalissuearticles values(58 % and 60 %, respectively); at 20-day’s incubation. Type of auxin was influenced most on root quality insert ignore into journalissuearticles values(thickness, color, branching); than on their frequency and number.
Keywords : Allochrusa gypsophiloides, Turkestan soaproot, Saponin bearing plant, Adventitious shoot, Micropropagation