- International Journal of Secondary Metabolite
- Volume:7 Issue:4
- Investigation of Some Biological Activities of Extracts Centranthus longiflorus subsp. longiflorus
Investigation of Some Biological Activities of Extracts Centranthus longiflorus subsp. longiflorus
Authors : Elif AYAR, Nezahat KANDEMİR, Şevket KANDEMİR, Umut ÇELİKOĞLU, Önder İDİL
Pages : 253-265
Doi:10.21448/ijsm.648072
View : 13 | Download : 6
Publication Date : 2020-12-15
Article Type : Research Paper
Abstract :In this study, the effects of above and below-ground extracts of Centranthus longiflorus subsp. longiflorus plant, commonly found in Turkey, on antioxidant, antimicrobial and DNA damage were evaluated. Plant extracts were prepared by applying three different solvents insert ignore into journalissuearticles values(hexane, methanol and ethanol);. The antimicrobial activity tests of the extracts were performed using four different standard strains and one yeast. DPPH, total phenolic content calculation and CUPRAC methods were applied for antioxidant activity studies. Additionally, the effects of plant extracts on DNA damage were investigated using pBR322 plasmid DNA. According to the data obtained, especially the below-ground hexane insert ignore into journalissuearticles values(MIC value:375μg/mL); extract showed more antimicrobial activity than other plant extracts, and it was found to be more effective Gram negative bacteria. The highest antioxidant activity was determined in extracts prepared with above insert ignore into journalissuearticles values(IC50 value of methanol extract:4.5mg/mL); and below-ground insert ignore into journalissuearticles values(IC50 value of methanol extract:5.7mg/mL); methanol. The above insert ignore into journalissuearticles values(93,9 μg GAE/mL); and below-ground insert ignore into journalissuearticles values(96.9 μg GAE/mL); methanol extracts were seen to have high total phenolic content. It has also been observed that above-ground hexane and methanol extracts have no effect on pBR322 plasmid DNA, but other extracts affect pBR322 plasmid DNA in the direction of degradation or deformation. Especially, the extracts of the above and below-ground ethanol had the effect of completely eliminating the open ring form. Therefore, it was concluded that this taxon could be widely used in the treatment and prevention of oxidative stress-related diseases in the future.Keywords : Antimicrobial, Antioxidant, Centranthus longiflorus, pBR322 plasmid DNA