RP-HPLC-UV Analysis of the Phenolic Compounds, Antimicrobial Activity Against Multi-Drug Resistant Bacteria and Antioxidant Activity of Fruit and Seed of Diospyros lotus L.ctivity of Fruit and Seed of Diospyros lotus L.

Authors : Zeynep AKAR, Aylin KARAKURT, Funda OKUMUŞ, Sedanur CİNEMRE, Azer ÖZAD DÜZGÜN, Bülent AKAR, Zehra CAN

Pages : 237-246

Doi:10.21448/ijsm.714108

View : 23 | Download : 6

Publication Date : 2020-12-15

Article Type : Research Paper

Abstract :The object of the present study was to investigate the antioxidant, antimicrobial activity and phenolic compounds of fruit and seeds of Diospyros lotus L. The fruits consumed by humans as nutrients were harvested from the plants that grow naturally in the province of Trabzon-Akçaabat, Eastern Black Sea Region of Turkey. The antioxidant activities of fruit and seeds of Diospyros lotus were determined by using four methods insert ignore into journalissuearticles values(% DPPH radical scavenging activity, FRAP antioxidant power determination, CUPRAC reducing antioxidant activity and total phenolic content insert ignore into journalissuearticles values(TPC); in five different proportion of methanol-water extracts. All extracts of fruit and seeds of D. lotus analyzed through whole antioxidant analysis methods showed significant antioxidant activity. In addition, antimicrobial activity of fruit and seeds extracted with DMSO was determined against seven standard bacteria and three multi-drug resistance clinical strains. Although fruit extracts did not have the antimicrobial activity against bacteria, seeds showed antimicrobial effect to both standard strains insert ignore into journalissuearticles values(Gram-negative and Gram-positive); and antibiotic resistance clinical isolates insert ignore into journalissuearticles values(Klebsiella pneumoniae carrying blaKPC, Acinetobacter baumannii, harboring blaOXA-23 gene and resistance to colistin, and multi-drug resistant Pseudomonas aeruginosa);. MICs’ value of plant seed extracts for standard strains was 0.75-25 mg/mL and antibiotic resistant clinical bacteria were 12.5 and 25 mg/mL. Additionally, phenolic compounds in methanol extracts of fruit and seeds were also determined in by HPLC using 19 standards. Gallic acid and chrysin phenolic compounds were the major phenolic compounds in fruit and seeds, respectively.
Keywords : Antioxidant, Diospyros lotus, Multi drug Resistant Bacteria, Phenolic Compounds