In vitro production of tropane alkaloids from Brugmansia suaveolens

Authors : Tijen TALAS OĞRAŞ, Elif TAHTASAKAL, Selma ÖZTÜRK

Pages : 1-13

Doi:10.21448/ijsm.934222

View : 13 | Download : 7

Publication Date : 2022-03-10

Article Type : Research Paper

Abstract :For thousands of years, secondary metabolites have been utilized as medications, flavors, pesticides, and dyes. For the generation of valuable secondary metabolites, in vitro plant culture techniques have been viewed as beneficial alternatives to whole plants. Brugmansia suaveolens is an ornamental plant including anticholinergic agents which are employed in medicine. Callus induction was performed from leaf and internode explants cultured on Murashige and Skoog’s medium supplemented with different concentrations and combinations of plant growth regulators insert ignore into journalissuearticles values(PGRs); with 6 treatments. The highest callus induction response was obtained from the leaf-originated explants insert ignore into journalissuearticles values(73%); on the medium supplemented with 0.4 mg/L KIN and 0.2 mg/L NAA which produced friable callus in 4 weeks. The cell suspension culture of B. suaveolens was established in shake flasks using friable calli. The extraction protocol of tropane alkaloids was optimized, atropine and scopolamine were obtained efficiently. The data could provide technical support for the large-scale production of valuable alkaloids of B. suaveolens in vitro systems with improved strategies.
Keywords : Brugmansia suaveolens L, Atropine, Callus, Scopolamine, Suspension culture