A research paper on the immunomodulatory and anti-inflammatory activities of olive tree (Olea europaea L.) leaf

Authors : Seda BEYAZ, Özlem GÖK, Abdullah ASLAN

Pages : 348-359

Doi:10.21448/ijsm.1075283

View : 17 | Download : 6

Publication Date : 2022-09-26

Article Type : Research Paper

Abstract :Olive tree insert ignore into journalissuearticles values(Olea europaea L.); leaf is known to have a number of bioactive properties being antioxidant, antihypertensive, antiatherogenic, anti-inflammatory, antifungal, antiviral and antimicrobial. In this study, the immunomodulatory roles of Olive tree insert ignore into journalissuearticles values(Olea europaea L.); leaf against oxidative damage caused by carbon tetrachloride insert ignore into journalissuearticles values(CCl4); in Saccharomyces cerevisiae were investigated. In the study, four groups were formed; namely, insert ignore into journalissuearticles values(i); Control Group: Yeast only planted group; insert ignore into journalissuearticles values(ii); CCl4 Group: Group given CCl4 insert ignore into journalissuearticles values(15 mM);; insert ignore into journalissuearticles values(iii); Olive Tree Leaf Group: The group given olive tree leaf insert ignore into journalissuearticles values(10%);; and insert ignore into journalissuearticles values(iv); Olive Tree Leaf + CCl4 Group: Olive tree leaf insert ignore into journalissuearticles values(10%); + CCl4 insert ignore into journalissuearticles values(15 mM); given group. Cultures of Saccharomyces cerevisiae were grown at 30 °C for 1, 3, 5, and 24 hours. Malondialdehyde insert ignore into journalissuearticles values(MDA);, glutathione levels insert ignore into journalissuearticles values(GSH);, cell growth and catalase insert ignore into journalissuearticles values(CAT); activity measurements were determined by spectrophotometer. Total protein concentrations were determined by SDS-PAGE electrophoresis and the Bradford protein method. According to the results obtained; compared to the CCl4 group, cell growth insert ignore into journalissuearticles values(1, 3, 5 and 24 hours);, total protein synthesis, and GSH and CAT activities insert ignore into journalissuearticles values(24 hours); increased in olive tree leaf groups, while MDA level insert ignore into journalissuearticles values(24 hours); decreased. Thanks to its strong bioactive properties, olive tree leaf has been found to increase cell growth and total protein synthesis by decreasing CCl4 induced oxidative stress in Saccharomyces cerevisiae culture. It has been concluded that if the olive tree leaf is used regularly, it will be beneficial in eliminating many health problems.
Keywords : Olive tree leaf, Oleuropein, Oxidative stress, Polyphenol, Saccharomyces cerevisiae