Mineral contents, antimicrobial profile, acute and chronic toxicity of the aqueous extract of Moroccan Thymus vulgaris in rodents

Authors : Nidal Naceiri MRABTİ, Hanae Naceiri MRABTİ, Latifa DOUDACH, Zineb KHALİL, Mohamed Reda KACHMAR, Mouna MEKKAOUİ, Ryan SHEİKH, Emad M. ABDALLAH, Gokhan ZENGİN, Samiah Hamad AL-MİJALLİ, Abdelhakim BOUYAHYA, Moulay El Abbes FAOUZİ, Menana ELHALLAOUİ

Pages : 397-414

Doi:10.21448/ijsm.1106820

View : 10 | Download : 6

Publication Date : 2022-12-21

Article Type : Research Paper

Abstract :Moroccan flora is rich in plants used in traditional medicine, but the further scientific investigation is necessary. The aim of the research was to evaluate the nutritional content and antimicrobial activity of Moroccan Thymus vulgaris, as well as its possible acute and chronic toxicological effects on rodents. Inductively coupled plasma atomic emission spectroscopy insert ignore into journalissuearticles values(ICP-AES); was used to determine the mineral content. The antimicrobial activity was determined using a well-diffusion test, a minimum inhibitory concentration insert ignore into journalissuearticles values(MIC);, and a minimum bactericidal/fungicidal concentration insert ignore into journalissuearticles values(MBC/MFC); assay. Acute and chronic toxicity studies were conducted in vivo on mice and rats, respectively. Following that, haematological, serum-biochemistry, and histological investigations were performed. Moroccan Thyme was shown to be a source of numerous minerals which are necessary for health promotion. All antimicrobial testing, disc diffusion, MIC, and MBC tests revealed that thyme had potent antibacterial activity against all microorganisms tested. Staphylococcus aureus was the most susceptible bacterium, followed by Salmonella enterica and Escherichia coli. Additionally, thyme exhibited great antifungal efficacy against Candida albicans. The acute toxicity results indicated that the aqueous extract of T. vulgaris is almost non-toxic when taken orally. According to the chronic toxicity study, the extract is generally safe when taken orally over an extended period of time. The biochemical and haematological characteristics of the serum and blood were within acceptable limits, and histological examination revealed no abnormalities. In conclusion, the findings of this investigation, confirm the antimicrobial efficacy of the aqueous extract of Moroccan T. vulgaris and its safety for experimental animals.
Keywords : Thymus vulgaris, Mineral analysis, Toxicity, Antimicrobial, Rodents