Oxidative stress induced by fluorine in Xanthoria parietina (L.) Th. Fr.

Authors : Ouahiba BENHAMADA, Nabila BENHAMADA, Essaid LEGHOUCHİ

Pages : 124-136

Doi:10.21448/ijsm.1136546

View : 8 | Download : 5

Publication Date : 2023-03-26

Article Type : Research Paper

Abstract :In our work we were interested in the toxicity of fluorine on the various parameters of stress: chlorophyll, proteins, and antioxidant system in the lichen Xanthoria parietina insert ignore into journalissuearticles values(L.); Th. Fr., and for this purpose, lichen thalli were treated by sodium fluoride insert ignore into journalissuearticles values(NaF); at concentrations of 0, 0.5, 1.0, 5.0 and 10.0 mM, for time scale 0, 24, 48 and 96 h. The analysis results obtained revealed that all the parameters evaluated showed significant variations compared to those of the controls. From the analysis results obtained, it was noted that chlorophyll a insert ignore into journalissuearticles values(Ca);, chlorophyll b insert ignore into journalissuearticles values(Cb); and total chlorophyll insert ignore into journalissuearticles values(Ca+b); decreased correlating with exposure times to NaF insert ignore into journalissuearticles values(r = -0.785, p < 0.001; r = -0.955, p < 0.001; r = -0.899, p < 0.001, respectively);, with a significant increase of Ca/b ratio insert ignore into journalissuearticles values(p = 0.00572**); showing that Cb was more affected than Ca. However, hydrogen peroxide insert ignore into journalissuearticles values(H2O2); increased insert ignore into journalissuearticles values(r = 0.949, p < 0.001);. In correlation with NaF concentrations, Glutathione insert ignore into journalissuearticles values(GSH); increased insert ignore into journalissuearticles values(r = 0.969, p < 0.001);, while proteins decreased insert ignore into journalissuearticles values(r = -0.872, p < 0.001);. Furthermore, results showed that catalase activity insert ignore into journalissuearticles values(CAT); increased correlating with increasing exposure time of X. parietina to increasing concentrations of NaF. Long-term exposure insert ignore into journalissuearticles values(48 h -96 h); caused a significant decrease in GSH content insert ignore into journalissuearticles values(p = 0.02*); followed by total destruction at time 96 h.
Keywords : Fluorine, Chlorophyll, Proteins, Antioxidant system, Lichen