Characterization of ethylenediaminetetraacetic acid and acid tolerance of foodborne pathogenic bacteria

Authors : Reha AZİZOGLU

Pages : 311-318

Doi:10.31015/jaefs.2020.3.10

View : 8 | Download : 8

Publication Date : 2020-09-15

Article Type : Research Paper

Abstract :Foodborne pathogenic bacteria are subject to different stressful conditions due to process conditions, storage and composition of food. It is crucial to understand the survival characteristics of these bacteria to develop effective measures to limit or eliminate their survival in food. EDTA is a chelating agent and commonly used in food formulations for its function to prevent discoloration or flavor loss in food and to extend shelf life. Due to its common use in food industry, it is important to understand its antimicrobial function for possible interaction with other antimicrobials for elimination of foodborne pathogens. In this study, different foodborne pathogenic bacteria including two Gram-positive insert ignore into journalissuearticles values(Listeria monocytogenes and Staphylococcus aureus); and three Gram-negative insert ignore into journalissuearticles values(Escherichia coli O157:H7, Pseudomonas aeruginosa and Salmonella typhimurium); bacteria were characterized for their survival and growth in the presence of EDTA insert ignore into journalissuearticles values(0.01 and 0.05%); and under acidic condition insert ignore into journalissuearticles values(pH 5.0);. The presence of EDTA in the growth media caused Gram-positive and Gram-negative bacteria to become more susceptible to subsequent stressful conditions compared to control insert ignore into journalissuearticles values(p<0.05);. Gram-negative bacteria were more tolerant to acidic conditions as well as presence of EDTA compared to Gram-positive bacteria insert ignore into journalissuearticles values(p<0.05);. This study provides insight on survival characteristics of foodborne pathogenic bacteria against selected stress conditions they are exposed in food and highlights the antimicrobial function of EDTA in food formulations.
Keywords : Stress response, Foodborne pathogens, Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, EDTA, Acid tolerance