Synthesis, characterization and evaluation of antimicrobial activities of silver nanoparticles obtained from Rumex acetosella L. (Sorrel) plant

Authors : Necmettin AKTEPE, Hafize BÜTÜNER, Ayşe BARAN, M.firat BARAN, Cumali KESKİN

Pages : 522-529

Doi:10.31015/jaefs.2022.4.4

View : 20 | Download : 7

Publication Date : 2022-12-30

Article Type : Research Paper

Abstract :Rumex acetosella L. insert ignore into journalissuearticles values(sorrel); is a plant belonging to the Polygonaceous family and is a species that grows naturally across Turkey. In this study, the characterization of silver nanoparticles insert ignore into journalissuearticles values(AgNPs); obtained from the Rumex acetocella L. insert ignore into journalissuearticles values(RA); plant using the green synthesis method was performed and their antimicrobial activities were investigated. AgNPs were successfully synthesized in the first stage of the study using plant extract taken from plant samples collected from the natural growing environment. Characterization of synthesized AgNPs was performed using appropriate analytical methods insert ignore into journalissuearticles values(UV-vis, FT-IR, XRD, SEM-EDX, TEM, Zeta Potential and Zeta Sizer);. According to the analysis results, it was determined that AgNPs had a maximum absorbance at 476 nm wavelength, a pentagonal, hexagonal, and spherical appearance, a size of 29.16 nm, and a zeta potential of -9.88 mV. The antimicrobial activities of AgNPs were tested using the microdilution technique, in which Minimum Inhibition Concentration insert ignore into journalissuearticles values(MIC); values were determined on gram-positive Staphylococcus aureus, Bacillus subtilis and gram-negative Pseudomonas aeruginosa, Escherichia coli bacteria and Candida albicans fungus. It showed a very strong antimicrobial effect on C. albicans, S. aureus and P. aeruginosa. Consequently, AgNPs had stronger antimicrobial activity at low concentrations and when compared to commercial antibiotics.
Keywords : Rumex acetosella L, AgNP, Antimicrobial activity, Characterization, Green synthesis