Determination of effective surface sterilization protocol in in vitro tissue culture for Giant Snowdrop (Galanthus elwesii Hook) bulbs

Authors : Çağlar KAYA, Tolga SARIYER, Esra ŞAHİN

Pages : 345-348

Doi:10.31015/jaefs.2023.2.12

View : 50 | Download : 58

Publication Date : 2023-06-29

Article Type : Research Paper

Abstract :Giant Snowdrop insert ignore into journalissuearticles values(Galanthus elwesii Hook); is a species of snowdrop belonging to the Amaryllidaceae family. In this study, the deformation rates of the bulbs was calculated as % insert ignore into journalissuearticles values(percent);. For sterilization G. elwesii bulbs were sterilized at different concentrations insert ignore into journalissuearticles values(1, 3, 5, 7, 9, 11 and 13 %); of sodium hypochlorite insert ignore into journalissuearticles values(NaOCl); for 5, 10, 15, 20, 25 minutes. The bulbs were rinsed with sterile distilled water 3 times for 5 minutes and then transferred to the MS medium. Contamination rates in MS nutrient medium were determined as percent insert ignore into journalissuearticles values(%); after 7 days. Deformation was not observed in the bulbs sterilized with 1 and 3% sodium hypochlorite solution however 100% contamination was detected. The most effective surface sterilization was obtained by soaking in 9-11% sodium hypochlorite solution for 5 and 10 minutes. As the sodium hypochlorite concentration and the application time increased, the surface sterilization of the bulbs increased, but the deformation rate of the scaly leaves of the bulbs increased due to this increase.
Keywords : Giant Snowdrop, Bulb, Sterilization, Contamination, Deformation