Effects of L-NAME, DEXA and L-NAME+DEXA on Systemic Blood Pressure of Hypertensive Pregnant and Non-Pregnant Wistar albino Rats

Authors : Muharrem BALKAYA, Gülsüm BACAK

Pages : 78-85

Doi:10.30704/http-www-jivs-net.460833

View : 16 | Download : 6

Publication Date : 2018-12-31

Article Type : Research Paper

Abstract :Objective: NO-inhibition has no effect on blood pressure insert ignore into journalissuearticles values(BP); of some spontaneous hypertensive animals, but when combined with dexamethasone insert ignore into journalissuearticles values(DEXA);, it increases BP. The study compared effects of L-NAME and/or DEXA on systemic BP of spontaneously hypertensive pregnant and non-pregnant Wistar albino rats. Method : In two simultaneous experiments 62 female rats were used. All animals were mated for 7 days. Sperm positive insert ignore into journalissuearticles values(n1 = 33); and negative insert ignore into journalissuearticles values(n2 = 29); animals were each divided randomly into 4 groups. BPs were recorded in both experiments on the 15 th day from tails indirectly, and then animals were given Physiologic Saline insert ignore into journalissuearticles values(Controls);, L-NAME insert ignore into journalissuearticles values(150mg/kg/day);, DEXA insert ignore into journalissuearticles values(100µg/kgBW/day); or L-NAME+DEXA insert ignore into journalissuearticles values(150mg and 100-µg per kg BW/day); for consequent 5 days. At 19 th day, BPs were measured again, before applications. Then, animals put into individual metabolic cages for 24-h urine collection. Thereafter, blood was collected under ether anesthesia, animals were euthanized and necropsied. Weights of animals insert ignore into journalissuearticles values(BWs);, left kidneys, adrenal glands, and fetuses; food consumptions; 24-h urine volume; urinary proteins, blood glucose, and fetus numbers were determined. Data were analyzed by ANOVA and ANOVA for repeated measures. Results : In pregnant animals, L-NAME had higher BWs than DEXA and L-NAME+DEXA insert ignore into journalissuearticles values(P = 0.021 and P = 0.012, respectively);. In non-pregnant animals, DEXA reduced BWs significantly compared with controls insert ignore into journalissuearticles values(P=0.042);. Interventions influenced only the diastolic blood pressure of pregnant animals insert ignore into journalissuearticles values(P = 0.043);. The difference between DEXA and L-NAME+DEXA was significant insert ignore into journalissuearticles values(P = 0.044);. The effects of interventions on other variables varied according to whether animals are pregnant or not. Conclusion : L-NAME and/or DEXA did not influence BP in hypertensive rats.  
Keywords : Wistar albino rats, L NAME, dexamethasone