Determination of the pharmacokinetics of marbofloxacin in endotoxemic sheep

Authors : Devran COŞKUN, Orhan ÇORUM, Enver YAZAR

Pages : 42-42

View : 13 | Download : 5

Publication Date : 2020-03-31

Article Type : Research Paper

Abstract :Endotoxemia causes many pathophysiological changes in the body. These changes may alter the pharmacokinetics of antibacterial agents used in the treatment. Purpose of this research; a); to determine the effect of the pharmacokinetics of marbofloxacin in the healthy sheep, b); to determine the effect of endotoxemia treatment insert ignore into journalissuearticles values(ET); on the pharmacokinetics of marbofloxacin in the healthy sheep, c); to determine the pharmacokinetics of marbofloxacin in the experimentally induced endotoxemic insert ignore into journalissuearticles values(LPS, E. coli O55:B5); sheep treated with ET. Seven clinically healthy Merino sheep were used. The study was carried out according to a 3-cycle cross-pharmacokinetic design and a 15-day wash-out period was performed between the stages. In the study, respectively, marbofloxacin insert ignore into journalissuearticles values(MB); 10 mg/kg insert ignore into journalissuearticles values(IV);, MB + ET insert ignore into journalissuearticles values(lactated ringer + Dextrose 5% + sodium chloride 0.45% 20 mL/kg, IV, 20 ml/kg/h, dexamethasone 0.5 mg/kg, SC); and LPS insert ignore into journalissuearticles values(10 μg/kg IV infusion for 30 minutes); followed by MB + ET were administered. In all phases of the study, blood samples were collected before insert ignore into journalissuearticles values(0 hours, control); and after MB administration at 0.083, 0.167, 0.25, 0.333, 0.417, 0.5, 0.75, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 18, 24, 36, 48, 72 and 96 h. Plasma MB concentration was measured using high performance liquid chromatography-UV and noncompartmental pharmacokinetic parameters were calculated. When the MB + ET group was compared with the MB group, no difference was found insert ignore into journalissuearticles values(P>0.05); in the basic pharmacokinetic parameters insert ignore into journalissuearticles values(t1/2λz , MRT, AUC0-∞, ClT and Vdss);. When the LPS +MB + ET group was compared with the other two groups, it was found that t1/2λz and MRT were prolonged, AUC0-∞ increased and ClT decreased insert ignore into journalissuearticles values(P<0.05);. In conclusion, it may be stated that endotoxemia may change pharmacokinetic values of marbofloxacin.
Keywords : Endotoxemia, sheep, marbofloxacin