A comparative study of VITEK-2, Double Disc Synergy and Combined Disc Methods for detection of ESBL (Extended Spectrum Beta-Lactamase) production in Escherichia coli and Klebsiella pneumoniae strains

Authors : Mustafa YILMAZ, Öznur AK, Demet HACISEYİTOĞLU, Sevil ALKAN

Pages : 116-120

Doi:10.16899/jcm.954056

View : 15 | Download : 7

Publication Date : 2022-01-15

Article Type : Research Paper

Abstract :Aim: In this prospective study we aimed to compare the effectiveness of VITEK-2 insert ignore into journalissuearticles values(bioMérieux, France); automated system, double-disc synergy insert ignore into journalissuearticles values(DDST); versus combined disc test insert ignore into journalissuearticles values(CDT); in detecting the Extended-Spectrum Beta-Lactamase insert ignore into journalissuearticles values(ESBL); positivity in Escherichia coli and Klebsiella pneumoniae strains isolated from various clinical samples. Material and Method: E.coli and K. pneumoniae strains inoculated on Mueller Hinton Agar plate. Susceptibility tests were performed with the VITEK 2 insert ignore into journalissuearticles values(BioMérieux, France); system before. Afterward, EBSL positivity was investigated manually DDST and CDT. Minimal inhibitor concentration insert ignore into journalissuearticles values(MIC); results of three tests were compared with each other according to the European Committee on Antimicrobial Susceptibility Testing insert ignore into journalissuearticles values(EUCAST); criteria. Results: 184 E. coli and K.pneumoniae strains, elevated. 92.9% of 98 patients with VITEK 2 positive results were positive with combined disc and DDS method, 100% of the 86 patients with negative results of VITEK 2 were negative with combined disc and MDC. Conclusion: VITEK 2 was found to have a sensitivity of 100%, a specificity of 92.4%, a positive predictive value of 92.8% and a negative predictive value of 100%. VITEK 2 was found to be compatible with validation tests for ESBL positivity.
Keywords : Escherichia coli, Klebsiella pneumoniae, VITEK 2, Double Disk Synergy DDS, Test, Extended Spectrum Beta Lactamase, Combined disc test