Protective effect of different layers of onion extracts (Allium cepa L.) on markers of oxidative stress in erythrocytes

Authors : Syed RİZVİ

Pages : 171-177

View : 15 | Download : 7

Publication Date : 2013-08-14

Article Type : Research Paper

Abstract :Onions insert ignore into journalissuearticles values(Allium cepa L.); are the rich source of flavonoids, consisting mainly quercetin-3,4´-O-diglucoside insert ignore into journalissuearticles values(QDG); and quercetin-4´-O-monoglucoside insert ignore into journalissuearticles values(QMG);, as major flavonoids. In the present study, we report the in vitro protective effect of different layers of onion extract on biomarkers of oxidative stress and on free radical induced oxidative hemolysis in erythrocytes. Results show a significant protection of oxidative stress by onion extract on erythrocyte subjected to tert-butyl hydroperoxide insert ignore into journalissuearticles values(t-BHP); treatment, as evidenced by the decrease in malondialdehyde insert ignore into journalissuearticles values(MDA); and increase in reduced glutathione insert ignore into journalissuearticles values(GSH); content. Onion extracts also showed significant protection of the erythrocyte from oxidative hemolysis. Quercetin insert ignore into journalissuearticles values(at micromolar concentration); showed significant antioxidant effect in protecting erythrocytes from t-BHP induced oxidative changes. The results are more pronounced for outer layers as compared to inner layers, suggesting that the outermost living layers had higher antioxidant activities compared to innermost layers.
Keywords : Ion channels, cell biochemistry, biophysics, calcium signaling, cellular function, cellular physiology, metabolism, apoptosis, lipid peroxidation, nitric oxide synthase, ageing, antioxidants, neuropathy