Aggregates of α-synuclein in brain tissue homogenates measured by newly designed Multimer-PAGE techniques

Authors : Jumana SALEH

Pages : 8-8

Doi:10.37212/jcnos.584670

View : 11 | Download : 6

Publication Date : 2019-06-21

Article Type : Other Papers

Abstract :Atypical aggregation of α-synuclein in the brain is linked to Parkinson’s disease insert ignore into journalissuearticles values(PD); progression. Mutimers of α-synuclein may be important for the biological function of α-synuclein. Currently, reliable methods to compare α-synuclein multimer abundance in biological samples are limited and sophisticated. A new technique termed “multimer-PAGE,” was designed by Killinger and Moszczynska, which combines in-gel chemical cross-linking with several common electrophoretic techniques to measure the multimerization of α-synuclein in complex biological samples of brain tissue lysates. Using this technique, it was possible to compare the ratio of α-synuclein multimers between brain tissue samples without the need for specialized equipment. 
Keywords : Brain, Multimer PAGE techniques, Parkinsons disease, α synuclein