Antioxidative and Neurotherapeutic Effect of Ascorbic acid on Albino rats Induced with Traumatic Brain Injury

Authors : Ibrahim BULAMA, Nasiru SULEİMAN, Lawal BİLBİS, Abdullahi ABBAS, Nasiru JİNJİRİ, Yusuf SAİDU, Mohamad Aris MOHD MOKLAS, Che Norma MAT TAİB, Musa CHİROMA

Pages : 922-936

Doi:10.37212/jcnos.800233

View : 12 | Download : 8

Publication Date : 2020-10-12

Article Type : Research Paper

Abstract :Generation of reactive oxygen species insert ignore into journalissuearticles values(ROS); during traumatic brain injury insert ignore into journalissuearticles values(TBI); has been identified as an important factor that is responsible for disease progression and cell death, particularly in secondary injury process. Ascorbic acid insert ignore into journalissuearticles values(AA); is an exogenous antioxidant that can be used to quench ROS in neurodegeneration. Its antioxidant properties have been reported in some neurodegenerative conditions in rats. In the present study, we examined the neurotherapeutic effects of AA in TBI induced rats. Three groups of seven rats each were used for this study. Group I was induced with TBI and treated with AA insert ignore into journalissuearticles values(67.5 mg/kg orally);, group II was traumatized but not treated insert ignore into journalissuearticles values(TNT); while group III was neither traumatized nor treated insert ignore into journalissuearticles values(NTNT);. Treatment started 30 min after TBI and lasted for 21 days. Morris water maze insert ignore into journalissuearticles values(MWM);, Elevated plus maze, and Open field test were carried out. Antioxidant enzymes [insert ignore into journalissuearticles values(Superoxide dismutase insert ignore into journalissuearticles values(SOD);, Catalase insert ignore into journalissuearticles values(CAT); Glutathion peroxidase insert ignore into journalissuearticles values(GPx);] and their gene expression were analyzed. Malondialdehyde level, S100B concentration and histological studies were conducted. The treatment with AA improved learning and memory, locomotor function and decreased anxiety in the treated groups compared to group II. S100B was significantly insert ignore into journalissuearticles values(p<0.05); lowered in the treated group compared to the group II rats. Treatment with AA also decreases malondialdehyde level when compared to group II. There were increased SOD, CAT and GPx activities in the treated group when compared to the control group. These were in agreement with their gene expressions that are highly expressed in the same groups. Our results suggest that neuroprotective effects of AA in albino rats can be the result of reduced lipid peroxidation and enhanced antioxidant status which can be a good neurotherapeutic strategy.
Keywords : Ascorbic acid, , Neurotherapeutic, antioxidant, Neurological, Neurodegeneration, antioxidant,