Expression of HIF-1α, TFRC-1 and TIM-2 Relative mRNA Levels in PTZ-Kindling Model of Epilepsy

Authors : Hüseyin Emre DÜNDAR, Aslıhan ÖZTAŞ, Saliha Rabia ŞAHİN, Seher YİLMAZ, Furkan KOCABAŞ, Enes AKYÜZ

Pages : 947-954

Doi:10.37212/jcnos.829166

View : 13 | Download : 8

Publication Date : 2020-01-05

Article Type : Research Paper

Abstract :Epilepsy is a neurological disease characterized by abnormal electrical activity and recurrent seizures in the central nervous system insert ignore into journalissuearticles values(CNS);. Changes in hypoxia and iron metabolism can stimulate seizures through the CNS and cardiac system. Cardiovascular system disorders such as arrhythmias also accompany this process. It was aimed to examine the genes of TFRC-1 and TIM-2, which contribute to ion homeostasis by providing hypoxia inducible factor insert ignore into journalissuearticles values(HIF); -1 and intracellular iron flow, through the kindled model of pentylenetetrazol insert ignore into journalissuearticles values(PTZ);. HIF-1α, TFRC-1 and TIM-2 gene expressions were investigated in both brain and heart tissue by RT-PCR method. As a result of the data, TIM-2 expression significantly decreased in the brain insert ignore into journalissuearticles values(p<0.01); and cardiac ventricle tissue insert ignore into journalissuearticles values(p<0.05); in female rats. TFRC-1 gene expression decreased in female brain tissue insert ignore into journalissuearticles values(p<0.05);. Our findings suggest that TFRC-1 and TIM-2 gene modulation may have therapeutic potential in epilepsy patients. In addition, TFRC-1 and TIM-2 genes may contribute to ferroptosis and oxidative stress mechanisms known to be associated with the seizure process by regulating iron transfer into the cell. It is crucial to conduct new studies on behalf of the future to elucidate iron metabolism in epilepsy through the TIM-2 and TFRC-1 genes.
Keywords : Epilepsy, PTZ kindling model, HIF 1α, TFRC 1, TIM 2