Characterization of lymphocyte infiltration in molar pregnancies

Authors : Jabbar Salman HASSAN

Pages : 68-72

View : 10 | Download : 12

Publication Date : 2016-12-30

Article Type : Research Paper

Abstract :Background: In normal pregnancy around 30% of stromal cells in decidualised endometrium are leucocytes, lymphocyte subtypes may play a role in the pathogenesis of molar pregnancies. The aim of the study is t o investigate the pattern of infiltrating lymphocytes in paraffin embedded tissue sections obtained from molar pregnancies. Material and Methods: This study included thirty-five archived paraffin-embedded samples of molar pregnancies; divided into 16 incomplete and 19 complete hydatid mole. Then dual immunofluorescence staining was used for phenotyping of lymphocytic infiltrate insert ignore into journalissuearticles values(CD3- CD19); and insert ignore into journalissuearticles values(CD4-CD8);. Independent sample t-test was used to compare the mean cell counts between different study groups. Results: There is higher T cells insert ignore into journalissuearticles values(CD3); infiltrate in complete hydatid mole than normal placental tissue insert ignore into journalissuearticles values(p=0.003); and not significant with incomplete hydatid mole insert ignore into journalissuearticles values(p=0.089);. Plasma cells insert ignore into journalissuearticles values(CD19); were higher in both complete hydatid mole CHM insert ignore into journalissuearticles values(p=0.012); and incomplete hydatid mole   iCHM insert ignore into journalissuearticles values(p=0.013);. Cytotoxic cells insert ignore into journalissuearticles values(CD8); were none significant in all groups while Helper cells insert ignore into journalissuearticles values(CD4); were significantly higher in CHM insert ignore into journalissuearticles values(p=0.017); and iCHM insert ignore into journalissuearticles values(p=0.025);. Conclusion: The higher tissue infiltration with plasma cells and helper cells in both CHM and iCHM highlight the importance of these cells in molar pregnancy pathogenesis.
Keywords : Hydatid mole, lymphocytes infiltration, Complete hydatid mole, incomplete hydatid mole