Expression of BMP-4 in dentigerous cyst and ameloblastoma: Is it a differentiation measure?

Authors : Soudabeh SARGOLZAEİ, Dina MALEKİ, Maryam ZOHARY

Pages : 17-22

Doi:10.26650/eor.2022903844

View : 8 | Download : 8

Publication Date : 2022-01-31

Article Type : Research Paper

Abstract :Purpose: This study aimed to determine the expression of Bone Morphogenic Protein-4 insert ignore into journalissuearticles values(BMP4); in dentigerous cyst insert ignore into journalissuearticles values(DC);, unicystic-ameloblastoma insert ignore into journalissuearticles values(UA);, and Multicystic-ameloblastoma insert ignore into journalissuearticles values(MA);, and assess whether this marker can be a differentiation measure. Materials and methods: This study included 30 DC, 30 UA, and 30 MA blocks if the histopathologic diagnosis of the lesion was definitive, the clinical information and medical records were complete, and the microscopic slides and the paraffin block were available. Age, gender, and location of the lesion were recorded. The samples were analyzed after the immunohistochemical staining insert ignore into journalissuearticles values(Envision technique);. BMP-4 marker was evaluated and reported using Intensity Score insert ignore into journalissuearticles values(IS);, Proportional Score insert ignore into journalissuearticles values(PS);, and Total score insert ignore into journalissuearticles values(TS);. The data were analyzed using SPSS version 21.0. Kruskal-Wallis and Mann-Whitney U tests were applied at the significance level of 0.05. Results: In this study, DCs, UA, and MA had a significant tendency to occur in males compared to females insert ignore into journalissuearticles values(P<0.001, P<0.001, and P<0.001 respectively);, and in the mandible compared to the maxilla insert ignore into journalissuearticles values(P=0.02, P=0.024, and P=0.02 respectively);. The epithelial IS was significantly different among three lesions insert ignore into journalissuearticles values(P<0.001);. IS was higher in MA than UA and DC insert ignore into journalissuearticles values(P<0.001 and P=0.006, respectively);. The IS was not significantly different among the three lesions in connective tissue and around micro-vessels insert ignore into journalissuearticles values(P=0.3 and P=0.26 respectively);. The PS in the epithelium and connective tissue of DC, UA, and MA had no statistical difference insert ignore into journalissuearticles values(P=0.549 and P=0.540 respectively);. The epithelial TS was statistically different among DC, UA, and MA insert ignore into journalissuearticles values(P<0.001);. The TS was higher in UA than MA and DC insert ignore into journalissuearticles values(P=0.004 and P<0.001 respectively);. Conclusion: The expression of BMP-4 in the epithelium was higher in ameloblastoma compared to DCs. BMP-4 is a potential measure to differentiate different types of ameloblastoma and dentigerous cyst. The differentiation of these lesions is important as the right treatment plan changes according to the diagnosis. 
Keywords : Ameloblastoma, dentigerous cyst, BMP4, Histopathological Assessment, Staining