AĞIZIN PREMALİGN VE MALİGN LEZYONLARINDA NORMAL AĞIZ MUKOZASI İLE KARŞILAŞTIRMALI OLARAK AgNOR SAYI VE DAĞILIMININ ARAŞTIRILMASI-INVESTIGATION OF AgNOR COUNTS AND DISTRIBUTION PATTERN IN ORAL PREMALIGN AND MALIGN LESIONS IN COMPARISON WITH THE NORMAL ORAL

Authors : Özen DOĞAN, Meral ÜNÜR, Sel YILDIRIM, Vakur OLGAÇ, Gülsüm AK

Pages : 267-272

View : 14 | Download : 11

Publication Date : 2013-06-06

Article Type : Research Paper

Abstract :INVESTIGATION OF AgNOR COUNTS AND DISTRIBUTION PATTERN IN ORAL PREMALIGN AND MALIGN LESIONS IN COMPARISON WITH THE NORMAL ORAL MUCOSA ÖZET Ağızın liken planus insert ignore into journalissuearticles values(n=10);, lökoplaki insert ignore into journalissuearticles values(n=9);, epidermoid karsinoma insert ignore into journalissuearticles values(n=7); olgularından alınan doku örnekleri % 10`luk formaldehitte tesbit edildikten sonra parafin blokları hazırlanıp AgNOR boyaması yapıldı. AgNOR değerleri liken planusda x= 4,6 ± 1, lökoplakide x= 5,3 ± 1,2 ve epidermoid karsinomada it= 6,5 ± 1,1`dir. Değerlerin gruplar arasında yapılan istatistiksel karşılaştırmasında liken planus  ile lökoplakiler arasında anlamlı bir fark saptanamamıştır insert ignore into journalissuearticles values(p>0.05);. Liken planus ile epidermoid karsinomalar arasındaki fark istatistiksel anlamlılık taşırken insert ignore into journalissuearticles values(p<0.01);, lökoplakiler ile epidermoid karsinomalar arasındaki fark anlamsız bulunmuştur insert ignore into journalissuearticles values(p>0.05);. Bu premalign ve malign lezyonların AgNOR değerleri, normal ağız mukozasından elde edilen doku örneklerinde insert ignore into journalissuearticles values(n=4); saptanan AgNOR değerleri insert ignore into journalissuearticles values(x= 1.75 ± 0.5); ile karşılaştırıldığında istatistiksel anlamlılık saptanmıştır insert ignore into journalissuearticles values(Liken planus-normal doku p<0.01);. Preparatların histopatolojik incelemesinde premalign lezyonlarda artan nukleolar organizasyona, malign lezyonlarda çok sayıda nukleolus dışı AgNOR benekçiklerine rastlandı. Sonuçlar premalign lezyonların prognozunun saptanmasında AgNOR sayısı kadar dağılımının da önemli olduğunu AgNOR boyamasının yardımcı yöntem olarak kullanılabileceğini göstermiştir. Anahtar sözcükler: AgNOR, malign transformasyon, liken planus, lökoplaki, epidermoid karsinom. ABSTRACT Investigation of AgNOR counts and distribution pattern in oral premalign and malign lesions in comparison with the normal oral mucosa. The tissue samples obtained from oral lichen planus insert ignore into journalissuearticles values(n=10);, leukoplakia and epidermoid carcinoma insert ignore into journalissuearticles values(n=7); were fixated in 10% formaldehid and paraffin blocks of the specimens were prepared. The AgNOR values were found to bex= 4,6 ±1 in lichen planus, x= 5,3 ±1,2 in leukoplakia andx- 6,5 ±1,1 in epidermoid carcinoma. When the values were statistically compared among the groups no significant difference was found between lichen planus and leukoplakia insert ignore into journalissuearticles values(p>0,05);. While the difference between lichen planus and epidermoid carcinoma was statistically significant insert ignore into journalissuearticles values(p<0.01);, the difference between leukoplakia and epidermoid carcinoma was not significant insert ignore into journalissuearticles values(p>0.05);. Statistically significant differences were found, when the AgNOR counts in premalign and malign lesions were compared with the counts in the tissue sections prepared from normal oral mucosa insert ignore into journalissuearticles values(lichen planus-normal tissue p<0.01; leukoplakia-normal tissuep<0.01; epid.Ca. normal tissue p<0.01);. The histopathological investigation of the specimens showed increased nucleolar organization in premalign lesi ons and multiple AgNOR dots outside the nucleolus in malignant lesions. Results of our study emphisized that besides the AgNOR counts, its distribution pattern is equally important in the predilection of the prognosis of premalign lesions and therefore AgNOR test can be a useful supplementary method for the histoprognostic evaluation of such lesions. Key words: AgNOR, malign transformation, lichen planus, leukoplakia, epidermoid carcinoma.  
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