PHYTOCHEMICAL PROFILE AND INSECTICIDAL ACTIVITY OF ESSENTIAL OIL FROM FRESH AND DRIED LEAVES OF NIGERIAN GROWN Citrus meyerii

Authors : Lamidi Ajao Usman, Bolatito Eunice Olanipekun, Ayorinde Victor Ogundele, Kannike Abdurasak Musa

Pages : 207-218

Doi:10.18596/jotcsa.09331

View : 10 | Download : 4

Publication Date : 2016-07-12

Article Type : Research Paper

Abstract :Leaves of Citrus meyerii harvested fresh and dried for four consecutive days were separately hydro-distilled and yielded 0.11 – 0.24 %insert ignore into journalissuearticles values(w/w); of essential oils. Characterisation of the oils using Gas chromatography - mass spectrometry insert ignore into journalissuearticles values(GC-MS); revealed the predominance of hydrocarbon monoterpenoids 51.1 – 68.3%. Oxygenated monoterpenoids, hydrocarbon sesquiterpenoids and oxygenated sesquiterpenoids constituted insert ignore into journalissuearticles values(17.4 - 24.9%);, insert ignore into journalissuearticles values(12.2 - 19.8%); and insert ignore into journalissuearticles values(0.0 – 2.5%); of the oils respectively. Principal constituents of the oils were; 3-carene insert ignore into journalissuearticles values(10.1-30.7%);, α-pinene insert ignore into journalissuearticles values(1.0%-18.7%);, d-limonene insert ignore into journalissuearticles values(5.2-6.4%);, cis-β-ocimene insert ignore into journalissuearticles values(5.8-14.2%);, citronellal insert ignore into journalissuearticles values(5.4-6.8%);, and β-elemene insert ignore into journalissuearticles values(3.0-5.8%);. The oils were of 3-carene and α-pinene chemotypes. Oils that were of 3-carene chemotype were those from fresh and the leaves dried for one and four days while the oils from leaves dried for two and three days were of α-pinene chemotype.  Insecticidal activities of the oils were determined using contact toxicity test on Callosobruchus maculatus . Regardless of whether the leaves were fresh or dried, the oils were active against the insect. Interestingly, there was no significant difference in the activities of the oils against the insect.
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