Investigation of Antioxidant Activity of M.comminus L. from Turkey due to its Essential Oil Composition and Polyphenolic Content

Authors : Belma HASDEMİR, Hasniye YAŞA, Hülya ÇELİK ONAR, Ayşe Sergüzel YUSUFOĞLU

Pages : 427-438

Doi:10.18596/jotcsa.54346

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Publication Date : 2017-01-08

Article Type : Research Paper

Abstract :In this study, it is aimed to investigate the chemical composition of the essential oils, antioxidant activity, and polyphenol content of methanol extracts of M.comminus L. leaves and berries. M.comminus L. Plant was collected from Yalova, Marmara region of Turkey. Its essential oil was prepared by hydrodistillation in a Clevenger-type apparatus in 0.5%. The chemical composition of the essential oil was analyzed by gas chromatography insert ignore into journalissuearticles values(GC); and gas chromatography/mass spectrometry insert ignore into journalissuearticles values(GC–MS);, using two columns with stationary phases of different polarity insert ignore into journalissuearticles values(polar ZB-WaxMS/apolar ZB-5MS);. On both columns monoterpenes were determined to be the dominant compounds. The myrtenyl acetate, α-pinene, 1,8-cineole, linalool, and limonene were the remarkable substances. As polyphenol compounds, the flavanoids and anthocyanidins in leaves and berries were detected by HPLC and furthermore, their antioxidant activities were studied with DPPH,  Cuprac and Folin – Ciocalteu method of the methanolic extracts. 
Keywords : M comminus L, anthocyanidins, flavonoids, essential oil, antioxidant activity