The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels

Authors : Kadir Erol

Pages : 133-148

Doi:10.18596/jotcsa.287321

View : 10 | Download : 5

Publication Date : 2017-01-09

Article Type : Research Paper

Abstract :The separation and purification methods for the isolation of an important biomolecule calmoduline protein is extremely important. Among these methods, the adsorption technique is extremely popular, and the cryogels as adsorbents with the macro porous structure and interconnected flow channels cryogel they have are preferred in this field. In this study, the adsorption of calmoduline via Cainsert ignore into journalissuearticles values(II); immobilized poly insert ignore into journalissuearticles values(2-hydroxyethyl methacrylate-glycidyl methacrylate);, poly insert ignore into journalissuearticles values(HEMA-GMA);, cryogels through changing interaction time, calmoduline initial concentration and temperature conditions. For the characterization of cryogels, the swelling test, Fourier Transform Infrared insert ignore into journalissuearticles values(FT-IR); Spectroscopy, Scanning Electron Microscopy insert ignore into journalissuearticles values(SEM);, surface area insert ignore into journalissuearticles values(BET);, elemental analysis and ICP-OES methods were performed. Nicotinamide molecule was used as Ca insert ignore into journalissuearticles values(II); chelating agent and the adsorption capacity of the cryogels was estimated as 1.812 mg calmoduline / g cryogel. The adsorption models of the adsorption reaction were examined by the Langmuir and Freundlich isotherm models and was determined to be more appropriate for Langmuir isotherm model.
Keywords : Adsorption, calmoduline, cryogel, nicotinamide