Effect of collection time on Chemical composition and Antibacterial activity of Flower Essential oil of Ocimum canum (sims) grown in Nigeria.

Authors : Lamidi Ajao Usman, Abdulrauf Olaniyi Yussuf, Bola Kudirat Saliu, Bolatito Eunice Olanipekun, Nusirat Elelu

Pages : 149-164

Doi:10.18596/jotcsa.287322

View : 12 | Download : 5

Publication Date : 2017-01-09

Article Type : Research Paper

Abstract :Flowers insert ignore into journalissuearticles values(1000g); of Ocimum canum harvested at different time insert ignore into journalissuearticles values(7am, 10am, 1pm, 4pm and 7pm); in a day were separately hydrodistilled and yielded 0.19 - 0.27% insert ignore into journalissuearticles values(w/w); of essential oils.  GC, GC-MS analyses revealed that the oils were predominated by oxygenated monoterpenoids insert ignore into journalissuearticles values(51.2-74.4%);. Hydrocarbon monoterpenoids constituted 6.2-10.2% of the oils. Percentage composition of hydrocarbon and oxygenated sesquiterpenoids in the oils were in the range of 1.3-22.0%. The most abundant constituent of the oils was linalool insert ignore into journalissuearticles values(40.5-58.7%);. Other principal constituents were; limonene insert ignore into journalissuearticles values(0.6 –7.5%);, terpinen-4-ol insert ignore into journalissuearticles values(1.4–5.6%);, eugenol insert ignore into journalissuearticles values(4.4–8.9%);, geranyl acetate insert ignore into journalissuearticles values(0.2–4.9%);, α-trans-bergamotene insert ignore into journalissuearticles values(3.2–9.4%); and E-isoeugenol insert ignore into journalissuearticles values(4.1–5.5%);. The predominance of linalool in the oils showed that the oils were of linalool chemotype. Antibacterial activity of the oils was evaluated against Staphylococcus aureus and Escherichia coli using agar diffusion method. Irrespective of the time of collection of the flower, the oils were found to be active against the tested organisms. However, they are more active on Escherichia coli than Staphylococcus aureus . The activity of the oils on the organisms was concentration dependent.
Keywords : Ocimum canum, Chemotype, linalool, terpene synthase, antibacterial