DNA- Binding, Biological Activities, and Chemical Composition of Wild Growing Epilobium angustifolium L. Extracts from Canakkale, Turkey

Authors : Aysema SAYIK, Ayşe Sergüzel YUSUFOĞLU, Leyla AÇIK, Gülen TÜRKER, Betül AYDIN, Leyla ARSLAN

Pages : 811-840

Doi:10.18596/jotcsa.319789

View : 10 | Download : 5

Publication Date : 2017-08-03

Article Type : Research Paper

Abstract :The usage of Epilobium angustifolium L. in folk medicine against prostate and skin diseases encouraged us to investigate pBR322 DNA-binding ability, biological activities and chemical composition of wild growing Epilobium angustifolium L .’s methanolic, ethanolic and aqueous extracts from Canakkale, Turkey. DNA-binding activity was studied by agarose gel electrophoresis and chemical composition via GC, GC-MS, HPLC, LC-MS, ICP-MS and AAS. Literature survey indicates no data on pBR322 plasmid DNA interaction of Epilobium species which is an important criteria for being a potential anticancer agent. Antibacterial and antifungal effects were tested against 11 bacterias and 3 fungis and compared with ampicillin, chloramphenicol and ketokanozol by agar well diffusion techniques. Total phenolic content was found by Folin-Ciocalteu method and antioxidant activity was obtained by DPPH and Fe insert ignore into journalissuearticles values(II);-FZ insert ignore into journalissuearticles values(ferric-ferrozine);   methods. This plant is rich of flavonoids,   hormones,   fatty and phenolic acids. Si, K,   Mg, P and Mn were the main inorganic elements. Epilobium angustifolium L. with its high DNA-binding activity and rich chemical composition can be a good potential anticancer, antimicrobial and antioxidant prodrug.  
Keywords : DNA Binding activity, pBR322 plasmid DNA Binding, , Epilobium angustifolium L extracts, cancer therapeutic, chemical analysis¬¬¬