Electrochemical xanthine biosensor based on zinc oxide nanoparticles‒multiwalled carbon nanotubes‒1,4-benzoquinone composite

Authors : Berna Dalkıran, Ceren Kaçar, Pınar Esra Erden, Esma Kılıç

Pages : 317-332

Doi:10.18596/jotcsa.307414

View : 11 | Download : 6

Publication Date : 2017-09-01

Article Type : Research Paper

Abstract :Zinc oxide nanoparticles insert ignore into journalissuearticles values(ZnONPs);, multiwalled carbon nanotubes insert ignore into journalissuearticles values(MWCNTs); and 1,4-benzoquinone insert ignore into journalissuearticles values(BQ); dispersed in chitosan insert ignore into journalissuearticles values(CS); matrix were used to construct a xanthine biosensor. Xanthine oxidase insert ignore into journalissuearticles values(XOx); was immobilized onto BQ-MWCNTs-ZnO–CS composite modified glassy carbon electrode insert ignore into journalissuearticles values(GCE); using glutaraldehyde as the crosslinking agent. The parameters of the construction process and the experimental variables for the biosensor were optimized. The xanthine biosensor showed optimum response within 10 s, and the sensitivity was 39.4 μA/mMcm 2 at +0.25 V insert ignore into journalissuearticles values(vs. Ag/AgCl);. The linear working range of the biosensor was found to be 9.0×10 −7 - 1.1×10 −4 M with a detection limit of 2.1×10 -7 M. The biosensor exhibited good long-term stability and reproducibility. The presented biosensor was also used for monitoring the freshnesses of chicken and beef flesh.
Keywords : Xanthine, biosensor, zinc oxide nanoparticles, carbon nanotubes, mediator