Cytotoxic effects of Mannich bases via induction of caspase-3 pathway on human oral squamous cell carcinoma

Authors : Cem YAMALI, Halise İnci GÜL

Pages : 187-194

Doi:10.18596/jotcsa.817007

View : 12 | Download : 7

Publication Date : 2021-02-28

Article Type : Research Paper

Abstract :In anticancer drug research, there is a need for the synthesis of compounds with selective cytotoxicity compared to the drugs in the market. The current study aimed to determine the cytotoxicities of the bis Mannich bases 1-9 towards human oral squamous cell carcinoma insert ignore into journalissuearticles values(OSCC);. Mannich bases showed cytotoxicity in low micromolar in the range of 1.7-27 µM against OSCC cell lines. The compounds 5 with the highest potency selectivity expression insert ignore into journalissuearticles values(PSE); value insert ignore into journalissuearticles values(318.1); and 7 with the highest tumor selectivity insert ignore into journalissuearticles values(TS); values insert ignore into journalissuearticles values(TS1:11.2, TS2:15.8); showed promising selective cytotoxicity towards cancer cell lines. According to Western blot analysis, representative compound 7 induced the activation of caspase-3 in HSC-2 cells. These results may suggest that the apoptosis pathway may be one of the possible mechanisms of the actions and lead compound 7 can be subjected to further bioassays.
Keywords : Mannich, anticancer, apoptosis