Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, cytotoxicity and genotoxicity investigations

Authors : Seda CEYLAN, Burcu ALATEPELİ

Pages : 69-78

Doi:10.18596/jotcsa.825115

View : 12 | Download : 9

Publication Date : 2021-02-28

Article Type : Research Paper

Abstract :Cryogelation has become an advantageous method to obtain macro-porous materials with well-defined, interconnected pores for tissue engineering applications. Herein, polyvinyl alcohol and chitosan polymers insert ignore into journalissuearticles values(PVA-CHI); were used to produce cryogel scaffolds via cryogelation. Glutaraldehyde was used as a crosslinking agent and the effect of crosslinking amount on the properties of scaffolds investigated. Glutaraldehyde amount was divided into 5, 10, and 15% total amount of polymer concentration. The optimized pore morphology was obtained as a scaffold containing 5% glutaraldehyde amount. In addition to the FTIR, SEM, swelling, and degradation analyses, mechanical tests were performed to present the characterization properties of the cryogels. Direct and indirect cytotoxicity test and genotoxicity experiments were performed with Mouse Embryonic Fibroblasts insert ignore into journalissuearticles values(MEF);. In addition, cell morphologies on scaffolds were analyzed with SEM. The results showed that PVA-CHI based cryogels had no genotoxic and cytotoxic effects on MEF cells and have a potential for tissue engineering applications.
Keywords : chitosan, PVA, Cryogel, Genotoxicity