Assay of Tretinoin using Safranine-O as a Chromogenic Reagent in Bulk and Dosage Forms

Authors : Thuttagunta MANIKYA SASTRY, Venkata Nagalakshmi KİLANA, Rama Krishna KARİPEDDİ

Pages : 139-146

Doi:10.18596/jotcsa.1176621

View : 6 | Download : 6

Publication Date : 2023-02-28

Article Type : Research Paper

Abstract :A new, economical and accurate analytical technique was developed for the assay of tretinoin insert ignore into journalissuearticles values(TTN); in bulk and formulations. While developing the method, it was found that the reaction was carried out due to the formation of ion-pair association complex involving the carboxyl group in the side chain of the TTN with safranine-O. The colored species formined was stable up to 60 minutes. The optical density of color species was measured at 520 nm. All the variables were optimized. The linearity range lies for the developed method within the concentration ranges of 2-10 μg mL-1.The linear correlation coefficient insert ignore into journalissuearticles values(r); and molar absorptivity insert ignore into journalissuearticles values(ϵmax);values were found as 0.9999 and 1.66 x 104 L mol–1cm–1. Percentage recoveries were found from 99.2 ± 1.8 to 100.2 ± 0.7.The method was validated as per ICH guidelines.
Keywords : safranine O SFN O, , Bioactive compounds, tretinoin, safranine O SFN O, , Ion pair association complex, spectrophotometry