Development of Two Simple Spectrophotometric Methods to Assay Phenylephrine-HCl as Pure Form And in Its Drug Forms

Authors : Hanan HAMEED, Salim ALİ

Pages : 677-688

Doi:10.18596/jotcsa.1260666

View : 33 | Download : 61

Publication Date : 2023-08-30

Article Type : Research Paper

Abstract :This work includes the development of two spectrophotometric methods which are sensitive, accurate, stable, and has good recovery for the determination of phenylephrine-HCl as free form and in the pharmaceutical preparations. In the method insert ignore into journalissuearticles values(A);, phenylephrine is oxidized by potassium permanganate in a basic solution of sodium hydroxide and the bluish-green color of the resulting manganate insert ignore into journalissuearticles values(MnO 42- ); is measured at wavelength 610 nm, which is linearly proportional to the amount of phenylephrine-HCl. Method insert ignore into journalissuearticles values(B);, is involved the oxidation of phenylephrine-HCl by using an excess amount of N-bromosuccinimide in an acidic medium of hydrochloric acid solution, the remaining insert ignore into journalissuearticles values(unreacted); amount of N-bromosuccinimide is used to bleach indigo carmine dye and the absorbance of the blue color of the remaining dye is measured at the wavelength of 610 nm. which is directly proportional to the concentration of phenylephrine-HCl. The molar absorptivity coefficients of methods insert ignore into journalissuearticles values(A); and insert ignore into journalissuearticles values(B); are estimated and equal to 1.5722×10 4 and 5.5191×10 4 L/mol.cm, respectively. Beer’s law of the both methods are linear in the concentration ranges 0.2–8.0 µg/mL insert ignore into journalissuearticles values(method A); and 0.2–3.5 µg/mL insert ignore into journalissuearticles values(method B);. The relative standard deviation values of methods insert ignore into journalissuearticles values(A); and insert ignore into journalissuearticles values(B); are also found to be better than 0.0286 and 0.0114, respectively. The two proposed methods are applied to estimate phenylephrine-HCl in injection and drop.
Keywords : Indigo carmine, KMnO4, NBS, Oxidation, Phenylephrine HCl, Spectrophotometry