Exploring the Antibacterial Efficacy of Silver Nanoparticles Synthesized through Abiotic Stress-Induced Germinated Seeds of Vigna radiata: A Comparative Analysis

Authors : Tissamol Abraham, K. P. Theertha, Sachin K. Ashok, Jebin Joseph, T Sajini

Pages : 981-994

Doi:10.18596/jotcsa.1335103

View : 22 | Download : 67

Publication Date : 2024-08-30

Article Type : Research Paper

Abstract :The novel microwave-assisted green synthesis of silver nanoparticles (AgNPs) from stress-induced germinated seeds of Vigna radiata (VR) is explored in this research. AgNPs were successfully synthesized using abiotic stress-induced germinated seeds of VR, induced by salinity, drought, and heavy metals such as sodium chloride (NaCl), polyethylene glycol (PEG), and a chromium solution, respectively. The characterization of the synthesized AgNPs was performed using various techniques, including UV-visible spectrophotometer, dynamic light scattering (DLS), zeta potential, XRD, FT-IR, and FE-SEM. The concentration of AgNPs synthesized from Vr-NaCl, Vr-Cr, Vr-PEG, and Vr-DW followed the order Ag/Vr-DW > Ag/Vr-NaCl > Ag/Vr-PEG > Ag/Vr-Cr. Notably, the synthesized AgNPs exhibited significant antibacterial activity against Staphylococcus aureus bacteria. A comparative analysis of the antibacterial efficacy of AgNPs synthesized using different stress-induced VR seed extracts revealed that AgNPs from PEG stress-induced germinated seeds of VR displayed excellent antibacterial activity. These findings underscore the potential of stress-induced germinated seeds of VR as a promising resource for producing AgNPs with exceptional antibacterial properties, thereby opening avenues for the development of innovative antimicrobial agents.
Keywords : silver nanoparticles, Vigna radiata, microwave, green synthesis, stress induced germination, antibacterial