Chestnut honey as a complementary medicine: determination of antibacterial activity, heavy metal residue and health risk assessment

Authors : Mesut Ertan GÜNEŞ

Pages : 82-89

Doi:10.31797/vetbio.931144

View : 15 | Download : 9

Publication Date : 2021-08-31

Article Type : Research Paper

Abstract :In this study, residue levels for heavy metal content and antimicrobial effects of 30 chestnut honey samples obtained from member beekeepers of Yalova Bee Association are examined. After 30 honey samples are digested under high temperature and pressure in a microwave oven, their Arsenic insert ignore into journalissuearticles values(As);, Lead insert ignore into journalissuearticles values(Pb);, Cadmium insert ignore into journalissuearticles values(Cd);, Mercury insert ignore into journalissuearticles values(Hg); limits are determined by using ICP-OES. By using Agar gel diffusion method, 19 of the honey samples’ antibacterial activity is tested on strains of Staphylococcus aureus insert ignore into journalissuearticles values(ATCC 25923);, Escherichia coli insert ignore into journalissuearticles values(ATCC 25922);, E. coli O 157: H7, B.cereus insert ignore into journalissuearticles values(ATCC 6633);, S.typhimirium insert ignore into journalissuearticles values(NCTC 12416); and L.monocytogenes insert ignore into journalissuearticles values(ATCC 7644);. Arsenic, Lead, Cadmium and Mercury residue levels in all honey samples are determined to be below the designated limits by Turkish Food Codex Regulation insert ignore into journalissuearticles values(2011);. The non-carcinogenic health risk associated with heavy metal contamination in honey is found to be very low. None of the 19 honey samples show antibacterial effect on strains of L.monocytogenes. However, at 100% honey concentration, in all 19 honey samples antibacterial effect is observed on strains of S.typhimirium, E. coli O 157: H7, except for only one honey sample; Escherichia coli and B cereus. The antibacterial activity on these bacteria is recorded to be maintained at 50% honey concentration. At 25% honey concentration, the antibacterial effect show a decline in most of the honey samples.
Keywords : Chestnut honey, antibacterial effect, heavy metals, health risk