Conducting Polymer Based Enzyme Electrodes Fabricated by Invertase and Polyphenol Oxidase

Authors : Elif DOĞAN, Ayşe Elif BÖYÜKBAYRAM

Pages : 113-119

Doi:10.34088/kojose.790262

View : 19 | Download : 6

Publication Date : 2021-11-30

Article Type : Research Paper

Abstract :Novel carbon fiber enzyme electrodes were constructed and evaluated. Fabrication of electrodes was performed via electrochemical deposition of a conducting matrice composed of polythiophene and polypyrrole insert ignore into journalissuearticles values(PTh–PPy); onto carbon fiber substrates. The enzyme was entrapped into the matrix during electropolymerization. Resultant biosensors represented higher kinetic parameters, Vmax and Km, in comparison to PPy matrice, which are 2.471 ± 0.150 mol min1- electrode1- and 30.60 ± 5.30 mM for invertase, 0.056 ± 0.012 mol min1- electrode1- and 842.00 ± 37.50 mM for polyphenol oxidase respectively. Optimum pH and temperature of the immobilized enzyme within PTh–PPy composite indicates that this matrice provides a more protective environment. The detection limit insert ignore into journalissuearticles values(LOD); of polyphenols was obtained as 0.037 mg mL1-. Polyphenol oxidase enzyme electrodes were proved to be used for the determination of polyphenolic substances in real samples and the results were confirmed by the Folin-Ciocalteau method
Keywords : Carbon Fiber Electrode, Conducting Polymer, Enzyme Immobilization, Polyphenol Oxidase