Fluorescence Glucose Biosensors Assays Analysis and Novel Classifications: Frequency Range Specification for Medical Applications

Authors : Rajaa Naeem, Doğu Çağdaş Atilla

Pages : 96-108

Doi:10.34088/kojose.1266492

View : 68 | Download : 28

Publication Date : 2024-11-30

Article Type : Research Paper

Abstract :The use of luminous glucose sensing as a potential replacement for more traditional forms of glucose measurement has shown encouraging results. Investigation of the efficiency of fluorescence resonance energy transfer (FRET) in glucose sensing is being conducted, with particular focus on the effect of donor-acceptor arrangement. The findings of the experiments indicated that the FRET efficiency was around 50.4% when FITC was used as the acceptor and TRITC was used as the donor. However, the FRET efficiency increased to over 60% when FITC was employed as the donor and TRITC was utilized as the acceptor in the experiment. The significance of the donor-acceptor configuration for efficient energy transfer has been brought to light by the findings presented here. In the process of glucose sensing, the data suggest that FITC should be utilized as the donor, while TRITC should be employed as the acceptor. The engineering medical application of a FITC-TRICTC biosensor requires an excitation wavelength of 544 nm and an absorption wavelength of 516 nm, respectively. In addition to these requirements, you will also need an antenna for transmission that operates at 580 GHz and a wavelength of 551 for the excitation. This article will be an extremely helpful resource for researchers working in the field of fluorescent glucose sensing. The article elucidates the essential concepts of competitive binding and oxidation, both of which are crucial to the process.
Keywords : Fluorescence Glucose Sensing, FRET, Competitive Binding, Oxidation, Hydrogels