Stereological Examination of Parietal and Temporal Regions of the Brain in Rats Induced with Epilepsy with Pentylenetetrazole

Authors : Veysel Akyol, Gamze Çakmak

Pages : 187-200

Doi:10.53518/mjavl.1520971

View : 15 | Download : 14

Publication Date : 2024-12-25

Article Type : Research Paper

Abstract :The aim of this study was to investigate the parietal and temporal regions of the brain stereologically after epilepsy induced by pentylenetetrazole (PTZ). 20 healthy male Wistar Albino rats, 3 months old, weighing approximately 250 gr, were chosen for the study. The rats were divided into PTZ and control groups, 10 in each group. 80 mg/kg PTZ was mixed into 0.5 ml of 0.9% physiological saline and administered intraperitoneally as a single dose injection to the PTZ group. Behavioral changes specified in six stages were determined by following the Racine scale within a 30-minute observation period. After perfusion, the rats’ brains were removed. After tissue tracking, an average of 10 sections were obtained for each animal, with a thickness of 1/75 of 5µm. Sections were stained with Hematoxylin-Eosin. The preparations were photographed with a 1.25 objective. For each brain tissue, the total lobus parietalis, lobus temporalis, the grey matter volume and the total number of neurons in the lobus parietalis and lobus temporalis were calculated. Mann-Whitney U test was used for statistical analysis (IBM SPSS for Windows, ver.25) No statistically significant difference could be detected in the total lobus parietalis, lobus temporalis and the grey matter volume, volume values of the brain and the number of neurons determined between the groups (p>0.05). However, when evaluated according to groups, a significant difference was obtained in the volume ratio values, the grey matter volume/total lobus parietalis and the grey matter volume/total lobus temporalis ratio (p<0.05).
Keywords : Epilepsi, Parietal lob, Pentylenetetrazol (PTZ), Stereoloji, Temporal lob.