An in-vitro study on the effectiveness of different irrigation solutions against Enterococcus faecalis with/without Erbium laser activation.

Authors : Gülşah BALAN, Sadık KALAYCI, Ayşen YARAT, Serap AKYÜZ, Fikrettin ŞAHİN

Pages : 45-51

Doi:10.35333/ERD.2020.265

View : 19 | Download : 10

Publication Date : 2020-12-31

Article Type : Research Paper

Abstract :Objective: Questions have been raised on the safety of prolonged use of NaOCl. There is a demand on developing alternative agents and activation methods for effective irrigation in a short time. Our study compares sodium hypochlorite to different irrigants with and without laser activation to evaluate antibacterial efficiency against Enterococcus faecalis. Methods: Bacteria was inoculated into 54 human teeth and incubated for 28 days to build up biofilm. The teeth then were randomly divided according to the irrigation method into two; insert ignore into journalissuearticles values(1); syringe insert ignore into journalissuearticles values(S); and insert ignore into journalissuearticles values(2); Erbium laser activation insert ignore into journalissuearticles values(LA); groups. In each group, teeth were divided into 4 experimental subgroups to evaluate the antibacterial properties of four different solutions of saline insert ignore into journalissuearticles values(NaCl);, sodium hypochlorite insert ignore into journalissuearticles values(NaOCl);, sodium pentaborate pentahydrate insert ignore into journalissuearticles values(NaB); and deuterium depleted water insert ignore into journalissuearticles values(DDW);. For S subgroups the amount of applied irrigant solution was 10 mL and for LA subgroups it was 10 mL. Samples were taken from canal wall and colony forming units were determined by plate counting after 24 hours. The survival rates of the bacteria were calculated as data. Results: Both techniques were reduced the bacterial survivors and no statistically difference was found between S and LA insert ignore into journalissuearticles values(p>0.05);. As expected, NaOCl-LA found more effective than Saline-LA insert ignore into journalissuearticles values(p<0.05); but not superior than NaOCl-S insert ignore into journalissuearticles values(p>0.05);. NaB solution showed similar antibacterial effect as NaOCl insert ignore into journalissuearticles values(p>0.05); Conclusion: The most effective NaOCl solution was followed by NaB, DDW and Saline, respectively. Laser activation did not increase effectiveness of solutions compared to syringe irrigation.
Keywords : deuterium depleted water, Enterococcus faecalis, Erbium laser activation, sodyum pentaborat pentahidrat, sodium hypochlorite