Effıcacy of sperm washıng and swım-up methods ın augmentatıon of total functıonal sperm fractıon for dırect ıntraperıtoneal ınsemınatıon

Authors : B. SUNAR-AKBA§AK, T. CAMLİBEL

Pages : 125-130

View : 9 | Download : 3

Publication Date : 1990-08-01

Article Type : Research Paper

Abstract :Total Functional Sperm Fraction insert ignore into journalissuearticles values(TFSF); measurements were used to estimate the overall ability of males to achieve conception in 29 men from a group of infertile couples. TFSF is a more comprehensive criterion than the traditional semen measurements because it incorporates all important seminal parameters such as total cell count per ejaculate insert ignore into journalissuearticles values(TCE);. percentages of normal morphology insert ignore into journalissuearticles values(N); and motility insert ignore into journalissuearticles values(M);. and a measurement of progressive. straight-swimming spermatozoa insert ignore into journalissuearticles values(P); on a scale of 1-3. The application of sperm washing followed by swim-up procedure can improve TFSF while separating the motile, highly progressive spermatozoa from round cells, leucocytes, spermatids, contaminating microorganisms and anti-sperm antibodies. In a clinical trial of direct intraperitoneal insemination insert ignore into journalissuearticles values(DIPI);, TFSF measurements were used for the evaluation of male fertility potential after sperm purification. TFSF measurements were utilized to standardize the number of active, highly progressive and morphologically normal spermatozoa for each individual case in every cycle of DIPI treatment Results of the clinical trial showed that two out of six couples participating in DIPI were successful in achieving conception and having healthy babies.
Keywords : Sperm washing, swim up male infertility, direct intraperitoneal insemination