The role of GluN1 activated nitric oxide synthase in a rat model of post-traumatic stress disorder

Authors : Beycan Gözde AYHAN, Aslı AYKAÇ, Kutlay GÜR, Banu AYDIN, Ece SEÇGİN, İrem SEVEN, Hülya CABADAK, M Zafer GÖREN

Pages : 67-72

Doi:10.5472/MMJoa.2902.01

View : 17 | Download : 10

Publication Date : 2016-04-01

Article Type : Research Paper

Abstract :Objectives: Activation of neuronal nitric oxide synthase insert ignore into journalissuearticles values(nNOS); and interrelated alterations of calmodulin and ionotropic glutamate receptor insert ignore into journalissuearticles values(GluN1); levels are unknown in post traumatic stress disorder insert ignore into journalissuearticles values(PTSD);.Materials and Methods: Sprague-Dawley rats of both sexes were exposed to to dirty cat litter, and then placed on an elevated plus maze. An anxiety index was calculated and tissue samples from hippocampus and amygdala were prepered in order to detect calmodulin, NOS and GluN1 by immunoblotting.Results: The anxiety indices of the traumatized rats were markedly higher than those of the controls insert ignore into journalissuearticles values(p<0.05);. GluN1 and calmodulin levels were decreased in the dorsal hippocampus and amygdaloid complex of the traumatized rats. NOS expression increased significantly in both the amygdaloid complex and dorsal hippocampus where the increase was statistically more prominent in the amygdaloid complex insert ignore into journalissuearticles values(p< 0.001); than in the dorsal hippocampus of the traumatized rats insert ignore into journalissuearticles values(p<0.05);.Conclusion: Predator exposure in rats causes long-lasting anxiogenic effects associated with increases in NOS and decreases in GluN1 expressions in brain areas related to PTSD symptoms and excitotoxicity. The results suggest that excitotoxicity occurs through other mechanisms rather than GluN1 receptors.Keywords: Predator scent
Keywords : Saldırgan hayvan kokusu testi, nNOS