GENOTYPE ANALYSIS OF HEPATITIS B VIRUS IN THE TURKISH POPULATION USING RESTRICTION FRAGMENT LENGTH POLYMORPHISM PATTERNS

Authors : Pınar Uysal, Naz Oğuzoğlu, Ayşe Özer

Pages : 213-216

View : 11 | Download : 4

Publication Date : 2016-12-03

Article Type : Research Paper

Abstract :Objective: Six different HBV genotypes have been defined comparing the complete HBV genome. The distribution of genotypes among infected populations varies geographically. A relationship between HBV genotypes and the development of chronic liver disease has been proposed. Therefore, this study was designed to classify HBV genotypes in the Turkish population using restriction fragment length polymorphism insert ignore into journalissuearticles values(RFLP); analysis of the S gene region. Methods: The HBV DNA extracted from forty- eight serum samples were subjected to polymerase chain reaction insert ignore into journalissuearticles values(PCR); using primers from the S region of HBV genome. Restriction enzymes, Hph I insert ignore into journalissuearticles values(Genotype F);, Nci I insert ignore into journalissuearticles values(Genotype E);, Alw I insert ignore into journalissuearticles values(Genotype C);, Ear I insert ignore into journalissuearticles values(Genotype B);, and Nia IV insert ignore into journalissuearticles values(Genotype A and D);, were used for RFLP analysis of amplified product of HBV DNA. Results: In the 48 serum samples investigated, 3 different genotypes were detected. These were as follows; Genotype D: 41 insert ignore into journalissuearticles values(85.41%);, Genotype A: 5insert ignore into journalissuearticles values(10.41%);, and Genotype F: 2 insert ignore into journalissuearticles values(4.16%);. Conclusion: This study shows a molecular heterogeneity of HBV in the Turkish population with at least 3 different genotypes observed in the 48 samples studied. The genotype D was by far the most common genotype in the Turkish population as is the case in all other Mediterranean countries. Key Words: Hepatitis B virus, Genotypes, S gene, Restriction fragment length polymorphism.
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