The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor

Authors : Didem KART

Pages : 137-143

Doi:10.5472/marumj.637153

View : 10 | Download : 7

Publication Date : 2019-10-31

Article Type : Research Paper

Abstract :Objectives: Interspecies interactions in poly-species biofilm are substantial. Our aim is to set up dynamic biofilm models of Enterococcus faecalis and Proteus mirabilis using Drip Flow Biofilm Reactor insert ignore into journalissuearticles values(DFR); and to evaluate the effect of these dual population on anti-biofilms of some antimicrobials. Materials and Methods: E.faecalis and P.mirabilis biofilms were formed in a DFR. Influences of the dual interactions on their susceptibilities to antimicrobial agents insert ignore into journalissuearticles values(disinfectants, antibiotics and probiotic strains); were determined. Results: Gluteraldehyde and quaternary ammonium compounds insert ignore into journalissuearticles values(QAC); effectively killed the cells in both biofilms of E.faecalis and P.mirabilis . However, the efficacy of hydrogen peroxide insert ignore into journalissuearticles values(H2O2); was dependant on the microbial species present. P. mirabilis was less susceptible to the ampicillin and ciprofloxacin in co-culture compared to when cultured alone. Here, the influence of the presence of E.faecalis on P.mirabilis susceptibility was determined. For high concentrations of ciprofloxacin insert ignore into journalissuearticles values(1024 and 512 μg/ml);, the log reduction in P.mirabilis cells was determined as approximately 4.5 and 3.5 in mono and dual-species biofilms respectively. Compared to B.lactis, L.acidophilus was found to be more effective both on single and dual species. Conclusion : The effect of antimicrobial agents on microbial cells in a polymicrobial biofilm may depend on the composition of the biofilm.
Keywords : Polymicrobial biofilm, E faecalis, P mirabilis, Antimicrobial, Drip flow reactor, Probiotic strains