Dental follicle mesenchymal stem cells regulate responses in sepsis

Authors : Fethi GUL, Deniz GENC, Mustafa Kemal ARSLANTAS, Noushin ZIBANDEH, Leyla TOPCU, Tunç AKKOC, İsmail CINEL

Pages : 7-16

Doi:10.5472/marumj.681996

View : 14 | Download : 5

Publication Date : 2020-01-31

Article Type : Research Paper

Abstract :Objective: Sepsis-induced immune alterations are associated with secondary infections and increased risk of death. The use of mesenchymal stem cells insert ignore into journalissuearticles values(MSCs); has been described as a novel therapeutic strategy. We evaluated the immunomodulatory effects of human dental follicle insert ignore into journalissuearticles values(DF-MSCs); on lymphocytes of sepsis and septic shock patients. Materials and Methods: Peripheral blood mononuclear cells insert ignore into journalissuearticles values(PBMCs); were isolated from venous blood samples of sepsis, septic shock and healthy subjects. PBMCs were co-cultured in the presence and absence of DF-MSCs with or without interferon-gamma insert ignore into journalissuearticles values(IFN-γ); for 72 hours. CD4+CD25+FoxP3+regulatory T insert ignore into journalissuearticles values(Treg); cell frequency, lymphocyte proliferation, cytokine levels and apoptosis were evaluated via flow cytometry. Results: DF-MSCs significantly suppressed proliferation of lymphocytes in sepsis group compared to septic shock group insert ignore into journalissuearticles values(p<0.005);. DF-MSCs remarkably increased Treg ratio in sepsis compared to control group insert ignore into journalissuearticles values(p<0.05);. Reduction of lymphocyte apoptosis in cocultures of DF-MSCs and PBMC was significant in both sepsis and septic shock groups. IFN-γ stimulation of DF-MSCs ameliorated shift in the T-cell subsets from Th2 to Th1 phenotype in septic shock. Conclusion: Our findings revealed that DF-MSCs have immunoregulatory effects both in sepsis and septic shock, by reducing interleukin-4 insert ignore into journalissuearticles values(IL-4); and increasing IFN-γ levels. This immunoreactivity regulation may open new therapeutic approaches for septic shock patients.
Keywords : Sepsis, Septic shock, Dental follicle mesenchymal stem cells, Immunomodulation