Evaluation of different respiratory samples and saliva for the detection of SARS-CoV-2 RNA

Authors : Rabia CAN SARINOGLU, Deniz GUNESER, Buket ERTURK SENGEL, Volkan KORTEN, Aysegul KARAHASAN YAGCI

Pages : 51-56

Doi:10.5472/marumj.866658

View : 15 | Download : 6

Publication Date : 2021-01-29

Article Type : Research Paper

Abstract :Objective: We aimed to analyse the positivity rate and cycle threshold values indicating viral loads for SARS CoV-2 among different respiratory specimens. Additionally, we evaluated the diagnostic efficacy of saliva samples. Patients and Methods: We included combined oropharyngeal and nasopharyngeal swab insert ignore into journalissuearticles values(cONS);, sputum, and tracheal aspirate insert ignore into journalissuearticles values(TA); specimens of patients. Unpreserved saliva samples were collected prospectively from hospitalized patients within 72 hours of admission. SARS CoV-2 RNA was extracted by using Bio-Speedy viral nucleic acid buffer than RT-PCR was performed with Bio- Speedy COVID-19 qPCR detection kit. Results: Retrospective evaluation revealed SARS CoV-2 RNA in 19.66% of cONS insert ignore into journalissuearticles values(n: 5819);, 30.77% of sputum insert ignore into journalissuearticles values(n: 39);, 29.41% of TA samples insert ignore into journalissuearticles values(n: 34); from 4812 patients. In the majority insert ignore into journalissuearticles values(86.72%); of the samples, the first cONS sample was positive. Consecutive cONS and sputum/TA samples were investigated in 52 patients of whom 11 were positive with either of these samples. Saliva positivity was detected in 60% of cONS positive insert ignore into journalissuearticles values(n: 20); and 30% of cONS negative insert ignore into journalissuearticles values(n: 12); patients. Conclusion: Although, cONS samples show the greatest diagnostic guidance, repeated sampling from multiple sites of the respiratory tract increases the possibility of COVID-19 diagnosis. Saliva samples might be considered as an alternative specimen.
Keywords : SARS CoV 2, OVID 19, RT PCR, Upper respiratory samples, Saliva, Cycle threshold