Effect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells

Authors : F. Tutku AKSOY, Ayse Mine YILMAZ, Gokhan BICIM, A. Suha YALCIN

Pages : 39-45

Doi:10.5472/marumj.1244676

View : 11 | Download : 4

Publication Date : 2023-01-31

Article Type : Research Paper

Abstract :Objective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined their effects on MCF-7 cells. Materials and Methods: Whey proteins obtained from 6% whey powder were treated with hazelnut oil insert ignore into journalissuearticles values(HO); and olive oil insert ignore into journalissuearticles values(OO); at a protein to lipid ratio of 1:10 at 60 ̊C for 120 minutes. The protein derivatives formed with whey protein and HO or OO were applied to MCF-7 cancer cells and healthy fibroblasts. The effects of protein derivatives on cell viability, apoptosis, reactive oxygen species insert ignore into journalissuearticles values(ROS); production, wound healing, cell cycle phase distribution and cell cycle related proteins Akt and p21insert ignore into journalissuearticles values(Waf1/Cip1); expressions were investigated. Results: Cell viability decreased significantly after 24 h of incubation with WP:OO. The percentage of apoptotic or necrotic cells varied between 5-10% and no statistically significant effect was observed. There was no statistically significant difference in ROS production and colony formation between controls and WP:HO or WP:OO groups. Treatment of cells with WP:OO for 24 h significantly decreased cell migration compared to the control group. G2/M phase was significantly suppressed in WP:OO group compared to the control group. WP:OO also increased the expression of p21insert ignore into journalissuearticles values(Waf1/Cip1); significantly when compared with the control group. Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer treatment.
Keywords : Whey proteins, Oleic acid, Cell survival, Apoptosis, Cell cycle