YY1 and NFYA: Potential tr-KIT Specific Transcription Factors in Prostate Cancer

Authors : Sercan ERGÜN, Ferda ARI, Erdal BENLİ, Diler US ALTAY, Tevfik NOYAN, Havva ERDEM, Yeliz KAŞKO ARICI

Pages : 202-207

Doi:10.19127/mbsjohs.1001931

View : 16 | Download : 11

Publication Date : 2022-05-31

Article Type : Research Paper

Abstract :Objective: Via the use of an alternative promoter, a truncated c-KIT protein insert ignore into journalissuearticles values(tr-KIT); of 30-50 kDa is generated, lacking extracellular and transmembrane domains. Moreover, over-expression of tr-KIT, a stronger activator than c-KIT, appears to be specific to prostate cancer insert ignore into journalissuearticles values(PCa);. Also, Imatinib, a tyrosine kinase inhibitor, blocks the activity of full-length c-KIT but has no effect on tr-KIT in PCa. Tr-KIT has its own nuclear factor binding site. However, the transcription factors insert ignore into journalissuearticles values(TFs); binding to this region specific to tr-KIT are not known yet. This study was conducted to define the most potential TFs specific for tr-KIT via in silico analysis. Methods: Tr-KIT potential TF binding sequence was uploaded into Tfsitescan database. Five TFs with the highest potential binding to this sequence were selected. Transcriptomic data of LNCaP insert ignore into journalissuearticles values(PCa expressing tr-KIT);, PC3 insert ignore into journalissuearticles values(PCa not expressing tr-KIT); and RWPE-1 insert ignore into journalissuearticles values(normal prostate); cell lines insert ignore into journalissuearticles values(GSM1647378, GSE36022 and GSM738189, respectively); from Gene Expression Omnibus insert ignore into journalissuearticles values(GEO); database were compared for gene expression levels of pre-defined potential tr-KIT specific TFs using DESeq package of R-program. Finally, two TFs having higher expression levels in both LNCaP and PC3 compared to RWPE-1 and higher expression levels in LNCaP compared to PC3 were detected. Results: Five TFs having the highest potential were selected as: YY1, c-MYB, IL8, NFYA and TCF3. Via in silico analysis performed, it was found that YY1 and NFYA have the highest potential to be tr-KIT specific TFs in PCa, among them. Conclusion: YY1 and NFYA TFs may take a role in formation of tr-KIT in PCa.
Keywords : Prostate cancer, transcription factors, gene expression regulation