Amoebicidal and Cytotoxic Activity of Propolis collected from Different Regions in Turkey on Acanthamoeba castellanii Trophozoites

Authors : Zeynep KOLOREN, Ömer ERTÜRK, Zülal ATLI ŞEKEROĞLU, Ülkü KARAMAN

Pages : 312-324

Doi:10.19127/mbsjohs.1253511

View : 38 | Download : 47

Publication Date : 2023-05-31

Article Type : Research Paper

Abstract :Objective: The present study aimed to investigate the amoebicidal effects of propolis collected from different regions in Turkey on Acanthamoeba trophozoites. Method: The propolis was collected from different geographic sites insert ignore into journalissuearticles values(Van, Erzurum, Gümüşhane, Ordu, Rize, and Muğla); in Turkey. Different concentrations of propolis ethanolic extract insert ignore into journalissuearticles values(in quantities from 1, 2, 3, 4, 5, 6, 7, and 8 mg/mL); and the same volume of trophozoites in 100 μl culture were blended for the identification of the amoebicidal efficiency of propolis. Results: The growth of trophozoites stopped in Turkish propolis extracts with 50% inhibitory concentrations insert ignore into journalissuearticles values(IC50);/48h for 5 mg/mL extract solution. Propolis showed more potent inhibitory effects on Acanthamoeba trophozoites at concentrations of 7, 6, 5, 4, and 3 mg/mL for 72 h. Propolis extract substantially inhibited human bronchial epithelial cells, especially at higher concentrations insert ignore into journalissuearticles values(7, 8, and 16 mg/mL);. Propolis can kill Acanthamoeba trophozoites at a concentration insert ignore into journalissuearticles values(of 3-6 mg/mL); but is safe for human bronchial epithelial cells at the same concentrations after 72 h treatment, this paves the way for propolis to be an alternative source of therapeutic drugs in the treatment of Acanthamoeba spp. Conclusion: Acanthamoeba infection still cannot be treated with drugs. In this study, propolis collected from different regions of Turkey showed amoebic and cytotoxic activities. Propolis extract, which is a natural product that can be used against Acanthamoeba trophozoites can be an alternative source of therapeutic drugs in the treatment of Acanthamoeba, supported by in vivo studies.
Keywords : Acanthamoeba sp, amoebicidal activity, Turkish propolis, MTT assay, Cytotoxicity, RP HPLC