OUT-OF-MIND INHIBITORS OF HUMAN SERUM PARAOXONASE 1 (PON1): AN IN VITRO STUDY

Authors : Mustafa Oğuzhan KAYA

Pages : 59-68

Doi:10.23884/mejs.2017.3.1.07

View : 13 | Download : 8

Publication Date : 2017-08-28

Article Type : Research Paper

Abstract :4-insert ignore into journalissuearticles values(dimethylamino);benzaldehyde, 3,5-dinitrosalicylic acid, berylliumsulfate tetrahydrate, urea, sodium azide, ironinsert ignore into journalissuearticles values(II);sulfateheptahydrate, 2-thiobarbituric acid, benzidine, and hydrazinium sulfate, which are commonly used in the pharmaceutical industry and medical studies, have been examined as novel type of inhibitors of PON1. PON1 was purified by hydrophobic column of Sepharose-4B-coupled L-tyrosine- 1-naphthylamine. PON1 enzyme activity towards paraoxon substrate was quantified spectrophotometrically. A critical overview of the effects of these nine reagents on PON1 which associated with cardiovascular diseases has been given. The IC 50 values were between 1.26×10 -4 M and 2.31×10 -4 M and benzidine showed the best inhibitory effect insert ignore into journalissuearticles values(IC 50 = 1.26×10 -4 M); for PON1 enzyme activity.
Keywords : Cardiovascular disease, PON1, Antioxidant activity, Inhibition, HDL C, LDL C