The in vitro cytotoxic, genotoxic, oxidative damage potential of enoxaparin sodium in human peripheral blood mononuclear cells

Authors : Kadri YILDIZ

Pages : 457-464

Doi:10.18621/eurj.781166

View : 12 | Download : 8

Publication Date : 2021-09-04

Article Type : Research Paper

Abstract :Objectives: Enoxaparin sodium, low-molecular weight heparin insert ignore into journalissuearticles values(LMWH); indicated for the prophylaxis deep vein thrombosis. As far as we know, its cytotoxic, genotoxic and oxidative effects have never been studied on any cell lines. The purpose of the present study is to evaluate the in vitro cytotoxic, genotoxic damage potential and antioxidant/oxidant activity of enoxaparin sodium on primary human whole blood cultures. Methods: After exposure to different doses insert ignore into journalissuearticles values(from 0.5 to 100 mg/L); of enoxaparin sodium, cell viability was assessed by the cytotoxicity tests including MTT insert ignore into journalissuearticles values(3, insert ignore into journalissuearticles values(4,5-dimethylthiazol-2);-2,5-diphenyltetrazolium bromide); and lactate dehydrogenase insert ignore into journalissuearticles values(LDH); release assays. The antioxidant activity was measured by the total antioxidant capacity insert ignore into journalissuearticles values(TAC); and total oxidative stress insert ignore into journalissuearticles values(TOS); parameters. To determine the genotoxic damage potential, the rate of chromosomal aberrations insert ignore into journalissuearticles values(CAs); and 8-oxo-2`-deoxyguanosine insert ignore into journalissuearticles values(8-oxo-dG); levels were also assessed. Results: Cytotoxicity assays showed that treatment with enoxaparin sodium caused significant decreases in the cellular viability in a clear dose-dependent manner. Also, it was found that enoxaparin sodium did not alter the TAC and TOS levels. The genotoxicity assay showed that the formation of CAs was not observed in the lymphocytes. Likewise, the levels of 8-oxo-dG did not change in treated cultures as compared to control values. Conclusions: Enoxaparin sodium appeared to exhibit cytotoxic but not oxidative and genotoxic damage potentials in cultured human blood cells.
Keywords : enoxaparin sodium, low molecular weight heparin, cytotoxicity, oxidative stress, DNA damage, human peripheral blood mononuclear cells